Gilson Connect
Oligonucleotides are core components of many molecular biology tools available today, including antisense gene therapy and primers for qPCR. Depending on the application, highly pure oligos are typically required, or experiments lack reproducibility — and for applications such as therapeutics, the consequences can be dire. To ensure that oligos are at the desired level of purity for their application, you need to use effective purification techniques. HPLC is one of the most effective and versatile approaches available for oligo purification, and in this article, we cover five tips to help improve your HPLC workflow to help you achieve highly pure oligos every time.
Synthetic oligonucleotides (oligos) are central to many cutting-edge biochemical applications across research and therapeutics. While solid-phase synthesis typically yields oligos with relatively high purity, they usually need further purification to avoid unintended side-reactions and interferences in downstream applications. Preparative HPLC is the preferred method for small to medium length oligos (10-60 bp). To increase productivity and maximize the profitability of your oligo research and development, finding the optimal LC purification platform is crucial.
Sample integrity is vital for accurate, trustworthy results and it can be affected by all stages of liquid handling, from pipetting to transport. Taking actions to improve pipetting and pipette storage, the lab environment, and sample storage and transport can protect your sample integrity and give you trustworthy results – learn more here.
Per- and polyfluoroalkyl substances (PFAS), or “forever chemicals”, are highly stable materials used in a variety of household products, industries, and processes. These chemicals can pose several health risks to living organisms and contaminate the environment. In this article, we elaborate on the above along with how automated solid phase extraction can be used to isolate PFAS from numerous matrices including water, soil, air (dust), and food such as seafood and eggs.
Quantitative polymerase chain reaction (qPCR) is a powerful and highly sensitive method of amplifying genetic material, with applications ranging from diagnostics and forensics to research.
However, ensuring the accuracy and reliability of your qPCR experiments can be challenging — even the smallest amount of contamination or failure to adhere strictly to liquid handling best practices can substantially impact your results.
So, what can you do to maximize your chances of delivering highly accurate qPCR results, time after time? In this article, we share our top 5 tips for qPCR success.
The manual cyclization step in a peptide purification workflow is time-consuming and can easily become the rate-limiting step of the whole workflow, leading to delays in peptide production. Automating a drop-wise cyclization process using a liquid handler with a large rack capacity and incorporating automatic orbital shaking is an ideal solution to this workflow bottleneck. Learn more in this technical note.
When choosing a high performance liquid chromatography (HPLC) system, it's important to consider how your future needs may evolve from your current needs. In this article, you’ll learn about the advantages of modular HPLC systems when it comes to current experimental needs and how the ability to swap out HPLC components safeguards the future of your system and your research.
In this technical note, the use of a PIPETMAX® 268 automated liquid handling system is described for the pipetting of the plasma from the whole blood sample. PIPETMAX can be used to aspirate a precise volume of the plasma and transfer this to a suitable analysis or storage tube. If the specific laboratory workflow will benefit from it, then diluent can also be added to the analysis tubes, and the plasma can be mixed into the diluent as part of the same method.
Preparative high performance liquid chromatography (HPLC) is a well-established method for purifying target compounds. Method development is usually done at a smaller scale to define sufficient resolution between the peaks without wasting large amounts of sample. It is common to scale-up the purification from a smaller column to a larger preparative one - learn more in this technical note.
How do you know you’re pipetting the volume you intend? Would you trust the results you’re getting if you didn’t know your pipettes were calibrated properly? Fortunately, there are standards that pipette manufacturers and calibration services follow so that you know you’re pipetting the correct volumes. Read more here.
