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This guide provides information about fraction collection techniques using TRILUTION® LC Software.
Many sample types, such as those from the environment or food, undergo testing to detect contaminating residues such as pesticides, antibiotics, chlorinated hydrocarbons, and compounds of health concern. While the exact governmental regulations surrounding testing methods differ by country, gel permeation chromatography (GPC) is one of the routine methods used to clean up samples before analysis. As a routine step in residue testing, is often a part of the workflow to prepare samples before analysis by a variety of chromatography methods such as GC, HPLC, or LC/MS. GPC removes unwanted substances such as lipids, proteins, cell debris, pigments, humic acid, and sulfur, that can interfere with downstream chromatography steps used to detect the residues. Including GPC cleanup in residue testing can help make chromatography columns last longer, protect instrumentation, and improve residue detection accuracy.
Plastics are ubiquitous in our society and are extensively used in a large variety of packaged foods. There is currently a great deal of interest in measuring the migration of these compounds into food products, as considerable uncertainty exists regarding the human health risks associated with exposure to such compounds. This application note describes the use of gel permeation chromatography (GPC) cleanup to separate a variety of plastic additives from a representative edible oil sample prior to analysis.
ICP-MS analysis is a technique of choice in many laboratories to quantify trace elements in many matrices. Automating this process reduces manual handling errors and provides traceability where manual preparation has none. This technical note shows how the use of a Gilson liquid handler and TRILUTION® LH Software makes it possible to automate those acidic dilutions prior to analysis by ICP-MS.
Peak-based fraction collection has the advantage that it’s based on real-time information. Your detector reveals a peak of interest, and the peak meets the criteria for collection. However, your molecules of interest are in the detector when you see the signal, and they need to pass through more tubing and parts before they can be collected. So how do you know when it is best to collect your sample? By knowing the fraction delay time.
Get fast, high-yield, and high-purity isolations without the need for long washing steps. This is possible thanks to our exclusive Exclusion-based Sample Preparation (ESP™) technology. It is perfect for capturing weakly bound protein complexes without subjecting them to high shear forces from pipette-based wash steps. Plus, it prevents contamination, dilution, or discarding of the original samples.
Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic compounds that have been widely used since the 1950s and are found in the environment and in the food supply. Because of the longevity of these chemicals, they pose a risk to human health through food and tap water consumption and other exposures. In order to understand the magnitude of the problem and to ultimately protect public health, regulatory agencies in various countries regularly detect and analyze PFAS in the food supply.
Chromatography plays a crucial role in the chemical and pharmaceutical industries. New drug innovation, pilot studies, and process development are a few examples of how it's used. Preparative chromatography is a method used to separate and purify target compounds derived from complex mixtures so that they can be used in other applications. When conducting these separations, there are certain dependencies that must be considered as scientists develop their methods. In this video, you'll learn more about these interdependent relationships involved in chromatographic separations.
Fraction collection can be conducted in a variety of manners. Most commonly, however, fractions are collected either based on a simple function of time or more complex parameters set off by the signals from your detectors. Learn more in our helpful video, which highlights 8 common questions about fraction collection.
Preparative high performance chromatography (prep HPLC or LC) plays a crucial role in many industries. It is used to isolate and purify desired compounds, removing impurities and contaminants that can affect the quality and performance of the end product. In this video, we answer your questions about prep HPLC and how it can fit into your lab's purification workflows.
